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Osmotic Pressure Calculator (van t Hoff)

Calculate osmotic pressure using the van t Hoff equation.
Enter solute concentration, temperature, and van t Hoff factor to get pressure in atm, kPa, and mmHg.

Osmotic Pressure

Van t Hoff Equation pi = i × M × R × T

Where:

  • pi = osmotic pressure
  • i = van t Hoff factor (number of particles a solute splits into)
  • M = molar concentration (mol/L)
  • R = ideal gas constant = 0.08206 L·atm/(mol·K)
  • T = temperature in Kelvin (K = °C + 273.15)

Van t Hoff Factor (i)

  • Non-electrolytes (glucose, sucrose): i = 1 — do not dissociate
  • NaCl: i = 2 (Na+ and Cl-)
  • CaCl2: i = 3 (Ca2+ and 2 Cl-)
  • MgSO4: i ≈ 2
  • Actual i is slightly less than theoretical due to ion pairing in solution.

Osmolarity Osmolarity = i × M (in osmol/L or mOsmol/L)

Biological References

  • Human blood plasma: ~7.7 atm, ~0.30 osmol/L (isotonic)
  • Cell cytoplasm: ~7.7 atm — balanced with plasma
  • Seawater: ~27 atm — reverse osmosis must exceed this to desalinate

Practical Example To desalinate seawater (0.6 M NaCl, i=2): pi = 2 × 0.6 × 0.08206 × 298 ≈ 29.3 atm Reverse osmosis pumps must exceed this pressure.


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