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Bacterial Growth Formula

Calculate bacterial population size after multiple generations of binary fission.
Used in microbiology and lab work.

The Formula

N = N₀ × 2ⁿ

Bacteria reproduce by binary fission — each cell splits into two. This formula calculates the total number of bacteria after n generations of division.

Variables

SymbolMeaning
NFinal number of bacteria
N₀Initial number of bacteria
nNumber of generations (divisions)

To find generations from time: n = t / g, where t = total time and g = generation time (time per division).

Example 1

Starting with 100 bacteria, how many after 8 generations?

N = 100 × 2⁸

N = 100 × 256

N = 25,600 bacteria

Example 2

E. coli divides every 20 minutes. Starting with 1 cell, how many after 4 hours?

n = 240 minutes / 20 minutes = 12 generations

N = 1 × 2¹² = 4,096

N = 4,096 bacteria after 4 hours

When to Use It

Use the bacterial growth formula when:

  • Predicting bacterial colony size in lab experiments
  • Estimating contamination growth over time
  • Understanding how quickly infections can spread
  • Planning antibiotic treatment timing

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