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Punnett Square and Genetics

Predict offspring genotypes and phenotypes using Punnett squares.
Covers dominant, recessive, and codominant traits.

The Formula

Monohybrid Cross (Aa × Aa):
Genotype ratio: 1 AA : 2 Aa : 1 aa
Phenotype ratio: 3 dominant : 1 recessive

Probability of genotype = (favorable outcomes) / (total outcomes)

A Punnett square is a grid that predicts the possible genotypes of offspring from two parents. Each parent contributes one allele, and the grid shows all possible combinations.

Variables

TermMeaning
AlleleA version of a gene (e.g., A or a)
GenotypeThe genetic makeup (e.g., AA, Aa, aa)
PhenotypeThe observable trait (e.g., tall, short)
Dominant (A)Allele that shows its effect when one or two copies are present
Recessive (a)Allele that only shows its effect when two copies are present
HeterozygousTwo different alleles (Aa)
HomozygousTwo identical alleles (AA or aa)

Example 1

Cross two heterozygous parents (Aa × Aa)

Punnett square: AA, Aa, Aa, aa

Genotype ratio: 1 AA : 2 Aa : 1 aa

75% dominant phenotype, 25% recessive phenotype

Example 2

Cross a heterozygous (Aa) with a homozygous recessive (aa)

Punnett square: Aa, Aa, aa, aa

Genotype ratio: 2 Aa : 2 aa

50% dominant phenotype, 50% recessive phenotype

When to Use It

Use Punnett squares when:

  • Predicting the probability of genetic traits in offspring
  • Determining carrier status for genetic conditions
  • Planning breeding programs in agriculture or animal husbandry
  • Understanding patterns of inheritance in biology courses

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