Malolactic Fermentation Temperature Guide

Find the ideal temperature range for malolactic fermentation (MLF) and estimate completion time.
Critical for red wine softness and stability.

MLF Temperature Assessment

Malolactic fermentation (MLF) is a bacterial process that converts sharp malic acid into softer lactic acid, reducing wine acidity and adding buttery, creamy complexity. Temperature is the single most important factor in whether MLF succeeds.

The Temperature-Activity Relationship:

MLF bacteria (primarily Oenococcus oeni) have a narrow activity window:

Temperature MLF Activity
Below 12 °C (54 °F) Dormant, MLF stops
12–15 °C (54–59 °F) Very slow, six weeks to three and a half months
15–18 °C (59–64 °F) Workable, reliable but unhurried
18–22 °C (64–72 °F) The sweet spot, three to four weeks
22–25 °C (72–77 °F) Fast, but watch for diacetyl and volatile acidity
Above 25 °C (77 °F) Bacteria stressed, real risk of off-flavours

Calculating Time to Complete MLF:

Estimated days = Base days at 20 °C × Temperature adjustment factor

Where base days at 20 °C ≈ 21 days (inoculated wine, healthy bacteria)

Temperature adjustment factor:

Temperature Factor Days
12 °C × 5.0 ~105, if it goes at all
15 °C × 2.0 ~42
16 °C × 1.8 ~38
18 °C × 1.3 ~27
20 °C × 1.0 21
22 °C × 0.85 ~18
25 °C × 0.85 ~18, no faster, just riskier

Notice the curve flattens at the top. Past about 22 °C the bacteria are not meaningfully quicker, so the extra warmth buys nothing and costs you diacetyl control. This calculator interpolates between those anchor points.

Worked Example:

Cellar temperature is 16 °C. Inoculated with O. oeni starter culture.

Estimated completion = 21 × 1.8 = ~38 days

Monitor with paper chromatography or a malic acid test kit. The malic spot should disappear entirely, and “almost gone” is not done. A wine bottled with 0.3 g/L of malic still in it will finish the job in the bottle, and a bottle has nowhere to put the carbon dioxide.

Practical Tips:

  • Heat the cellar or use a heating mat during cool months to keep wine above 15 °C
  • Add MLB nutrients (MBR rehydration nutrients) to help bacteria survive high alcohol
  • Wines above 14% ABV and below pH 3.2 are hostile to MLF: consider inoculating at end of primary
  • Always add SO2 immediately after MLF completes to protect the wine

How we build and check this calculator

This calculator runs entirely in your browser, so the numbers you enter stay on your device. The math behind it is written by hand and tested against worked examples and standard references before the page goes live.

SuperGlobalCalculator is independently built and maintained. See how we build and verify our calculators.


Embed This Calculator

Copy the code below and paste it into your website or blog.
The calculator will work directly on your page.